C3P3 is the first ever entirely artificial eukaryotic expression system. that generates mature messenger RNA (mRNA) in the cytoplasm of eukaryotic cells (i.e. organisms with cells having a cell nucleus enclosed within membranes), including Humans and other mammals.

The Human Embryonic Kidney 293 cell line (HEK-293 cells) is emerging as important cell line, which is used for the production of nearly 15% therapeutic proteins. HEK-293 is also the preferred cell line for bioproduction of recombinant therapeutic viruses, especially lentiviruses. The C3P3 bioproduction tools adapted to the HEK-293 line are under development and particularly appropriate for the bioproduction of recombinant viruses.

When comparing transient expression with the C3P3 system with standard transient expression vectors, the protein yield by transient transfection is greater of 5-fold. Moreover, any proteins tested so far can be expressed with the C3P3 system. The first generation of the C3P3 system has also demonstrated impressive performances for the production of recombinant orthoreoviruses, with production yields 1,00-fold greater than the system classically used.

Titers of recombinant orthoreovirus produced in BHK cells transfected with plasmid under control of the standard expression system (T7 RNA polymerase system) of C3P3-G1, together with the four reovirus reverse genetics plasmids that together express all 10 reovirus genes. Adapted from Eaton, Kobayashi et al. J Virol 2017, 91: e02416.
An advanced prototype of this cell lines is already available. However, the final version of C3P3 HEK-293 bioproduction tools will be only available early 2019. These cells will be provided with the corresponding backbone plasmids for protein or lentiviral bioproduction. The target gene can be inserted in the backbone of the plasmids by the end user. Alternatively, we can design any gene with Eukarÿs proprietary algorithm, clone it into our backbone construction and provide the final plasmids.