Bioproduction of recombinant viruses
Our first bioproduction tools are more specifically designed for the production of recombinant virus.
The Human Embryonic Kidney 293 cell line (HEK-293 cells) and Baby Hamster Kidney 21 (BHK-21 cells) are used increasingly for the production for the production of nearly 15% therapeutic proteins. These cells lines are also widely used for the bioproduction of recombinant viruses, especially lentiviruses.
When comparing protein production by transient expression using the C3P3 system to standard transient expression vectors, yields up to 5-fold greater can be synthesized. The first generation of the C3P3 system has also demonstrated impressive performances for the production of recombinant orthoreoviruses, with production yields 100-fold greater than the system classically used.
An advanced prototype of this cell line is available with its final version of expected mid-2019. These cells will be supplied together with the backbone plasmids for recombinant protein or virus bioproduction. The target genes can be inserted in the backbone of the plasmids by the users. Alternatively, we can design any gene construction and provide the constructions.
Titers of recombinant orthoreovirus produced in BHK cells by means of the C3P3-G1 technology in comparison to the standard expression system (T7 RNA polymerase). Adapted from Eaton, Kobayashi et al. J Virol 2017, 91: e02416.